Day 1 :
Keynote Forum
Lew P Christopher
Lakehead University, Canada
Keynote: Sustainable production of bioenergy and value-added products for the growing low-cost bio-resource economy
Time : 10:00-10:35
Biography:
Lew P Christopher holds a Master’s degree in Chemical Engineering, PhD degree in Biotechnology, and has more than 20 years of industrial and academic experience. Currently, he serves as Director of the Biorefining Research Institute at Lakehead University. His research mission is to add value to the emerging Bioeconomy by applying an integrated biorefinery approach to the development of renewable energy technologies. He is a Member of the Editorial Board of several international biotechnology journals, advisory boards, and professional societies. He has made over 400 scientific contributions to the field of bioprocessing of lignocellulosic biomass.
Abstract:
The global trend for production of bioenergy and bioproducts from renewable resources is currently steered by three important drivers: 1) diminishing reserves of readily recoverable oil and fluctuating oil prices; 2) growing food and energy needs; and 3) increasing greenhouse gas (GHG) emissions. The global production of plant biomass, over 90% of which is lignocellulose, is about 2 x 1011 tons per year, with up to 2 x 1010 tons of the primary biomass remaining potentially accessible and available for bioprocessing. Current estimates indicate that the global energy demand will continue to increase and reach 653 exajoules (EJ) in 2020) and 812 EJ in 2035. At a price of $107 per oil barrel, the cost of the lignocellulosic feedstock (US $2.6/GJ at $50/dry ton biomass) is lower than natural gas ($3.3/GJ) and crude oil ($17.2/GJ). However, at the current low oil prices, the cost of lignocellulose conversion ($20/GJ) exceeds nearly twice that of fossil fuels, which necessitates further optimization of the biomass conversion routes. Lignocellulosic biorefineries are the ultimate integrated biomass conversion facilities that are nowadays viewed as one of the major economic pillars of the emerging global Bioeconomy. However, less than 10% of the global fuels and chemicals production is currently biobased. This is mainly due to the fact that bioproducts are not yet cost-competitive to their petroleum-based counterparts. As the biomass feedstock comprises about 50% on average of the total production costs, it has now been recognized that low-value biomass and biomass waste streams can provide a cost-effective alternative to improve the economic viability of biorefineries. Among other, this approach offers two major advantages: 1) significantly lower bienergy production costs; 2) significantly reduce waste treatment costs, carbon footprint and GHG emissions. This presentation will discuss opportunities for valorization of industrial, agricultural and municipal biomass waste and related technological challenges that we need to overcome in our transition to a low-cost bioresource economy and biobased society.
Keynote Forum
Weilan Shao
Jiangsu University, China
Keynote: How to make a recombinant microbial strain of bio-safety for agricultural and industrial engineering?
Time : 10:35-11:10
Biography:
Weilan Shao holds a PhD Degree in Microbiology from the University of Georgia, USA (1993). She has been working as a Distinguished Professor in Jiangnan University, Nanjing normal University and Jiangsu University in China since 2000. Her research mission is to develop feasible and economic effective approaches for enzyme production and renewable bioenergy processing by using molecular biotechnology. She has published more than 100 papers and obtained 2 US patents and more than 15 Chinese patents.
Abstract:
Microbes have been widely applied to agricultural and industrial processes including food, forage or fertilizer fermentation, or industrial production of enzymes or fermentation products. These microbial strains usually should be improved by genetic mutagenesis to meet the requirement of their application. DNA recombinant techniques offer a fast, convenient and accurate approach for genetic modification of microbe strains. In a genetic modification system, a selection marker (SM) is essential for the functions of allowing transformants to be selected from non-transformed cells and maintaining recombinant properties by adapting to the selective stress of a culture medium. Drug-resistant genes are the most commonly used SMs in bacterial systems, however, they have a potential to spread antibiotic resistance to pathogenic microbes. Many currently available auxotrophy markers have been developed for yeast and fungus systems; however, these SMs require certain conditions to create selective stress which can be achieved in a laboratory, but not in environments such as biomass, forage, pulp, animal guts, wastewater, or soil. Therefore, bio safe and effective SM is required for the construction of recombinant strains used in agricultural and industrial engineering. Glutamine:fructose-6-phosphate aminotransferase (GFAT) catalyzes the formation of glucosamine-6-phosphate, and its gene are essential for microbes. The GFAT-deficient strains of bacteria, a fission yeast and an aspergillus have been constructed, and GFAT vectors can be selected and maintained stably in these cells. Using the GFAT-encoding gene can prevent the use of a drug-resistant gene as an effective SM in bacteria, yeasts and fungi. Another unique property of the GFAT-SM is that no particular compound is prohibited or required for creating a selective stress, i.e. a selection stress is naturally occurred in media or environments lacking exogenous glucosamine. Therefore, GFAT-SM will allow the release of genetically modified microbes from laboratory for practical applications in agricultural and industrial engineering.
Group Photo and Refreshments Break 11:10-1140 @ Sylt Foyer
Keynote Forum
Patrice J Mangin
BioEnergy La Tuque, Canada
Keynote: Forest residues biorefinery: a diversification potential for traditional petroleum industry
Time : 11:40-12:15
Biography:
Patrice Mangin holds a PhD in Process Engineering from Institut National Polytechnique (INP) of Grenoble and an Engineering Degree from INP Pagora, France. He is a bio-economy/bioenergy industry Chair holder, Professor, Chemical Engineering Department at the University of Québec at Trois Rivières (UQTR). He is the CEO of BioEnergy La Tuque (BELT) whose main objective is to build the first Canadian large scale biorefinery to convert harvest forests residues into renewable fuels (around 1 B$ project). He is an Associate Member of the Hydrogen Research Institute (IRH) and Interdisciplinary Research Center in Operationalization of Sustainable Development (CIRODD). He demonstrates over 40 years of experience with successful track record in the pulp and paper, forest products, and printing industries. He is on the Board of AQPER (Quebec association for the production of renewable energy), Chair of the biofuels and Member of the biomass committees. He is on the strategic committee of The Tuque Forest of Education and Research and on AGENDA 2020 (Washington) Chief Technical Officers Committee which coordinates the US strategy on the development of forest products and forest bio-economy. He also participates in the FPAC (Forest Products Association of Canada) BioPathWay.
Abstract:
Quebec, like other Canadian provinces, has access to millions of tons of renewable biomass in the form of harvest forest residues. The Quebec chief forester has evaluated that, for the forest area around La Tuque (area 04), the capacity of unused harvest residues ranges from 1.1 to 1.8 green metric tons per year; the larger figure includes foliage and needles but not the tree stump and root system, which are not considered as biomass in Quebec. To valorize such unused biomass, BioEnergy La Tuque (BELT) has undertaken a large-scale biorefinery project that aims at converting the renewable, unused forest residues into “drop-in” quality fuels. The forest-based biorefinery project being the first of the kind in Canada, BELT, as the project promotor, has initiated a thorough due diligence techno-economic evaluation to mitigate and de-risk the project and attract potential investors. BELT main objective is to provide a no-compromise optimum techno-economic solution for the refinery. The thorough evaluation of all project facets is required both to select the best available process line but also to validate the availability of biomass at a low cost and over a long period-of-time, i.e. 25 years, exceeding the amortizing period, usually 15 years in the petroleum industry, of such projects. The BELT project has presently the full support of both the Canadian federal and Quebec province governments, as there is a need for a political coherence between the renewable energy policy and regulations and the development of the renewable energy industry. Our experience proves the full feasibility of such a project. The presentation will describe the BELT project and use it as a case study to elaborate on all the techno-economic-political aspects required to successfully develop in Canada, and elsewhere in the world, the bioenergy industries.
Keynote Forum
Jung Chang Wang
National Taiwan Ocean University, Taiwan
Keynote: Thermal energy storage of alumina nanofluids using innovation dimensional analysis
Time : 12:15-12:50
Biography:
Jung Chang Wang is a Full Professor in the School of Marine Engineering, National Taiwan Ocean University, Keelung, Taiwan, and is also the Director of the Thermal-Fluid Illumination Laboratory. He received his Bachelor and Master Degrees from National Cheng Kung University and PhD (Mechanical) from National Taiwan University in Taiwan in 2007. He has been teaching and researching on electronic heat transfer and renewable energy for more than ten years. He has published more than 100 research papers in international journals, conferences and patents, and edited five book chapters. His main research interest includes applied and software engineering in thermal-fluid science.
Abstract:
A two-step synthesis preparing the thermoelectric nanofluids through an ultrasound technique was studied in the present article. The best mixing method was determined by a sedimentation experiment of suspendibility, stability, and thermal conductivity. 0.5-2.5 wt.% concentration thermoelectric nanofluids were added into a battery cell with copper and aluminum electrodes for an oxidation reduction reaction to test the generating capacity between 20 and 40°C. Two empirical formulas of thermal conductivity and generating capacity for thermoelectric nanofluids were derived by the innovation dimensional analysis. The results of the property verification and experimentation indicated that thermoelectric nanofluid and emulsifying agent mixture at a concentration of 1 wt.% had the best thermal conductivity, and that this decreases as the concentration increases. At 40°C, 2.5 wt.% thermoelectric nanofluid also had the highest electric charge density; however, the rate of increase was less than 7% higher than that for 2 wt.% thermoelectric nanofluid. The results also indicated that for 0.5-2.5 wt.% thermoelectric nanofluid between 20-40°C, inserting the temperature and concentration parameters can estimate the thermal conductivity and the electric charge density using the empirical formulas in the present study.
- Biomass feed stocks for renewable energy generation
Bioethanol
Bioenergy Applications
Energy and Environment
Session Introduction
Soo Young No
Chungbuk National University, Korea
Title: Application of biomethanol to advanced CI engines: a review
Time : 12:50-13:20
Biography:
Soo Young No has his expertise in atomization and sprays, combustion and emission characteristics in applying the liquid biofuels to internal combustion engines, particularly compression ignition engines. The review papers on liquid biofuels published by him include the biodiesel obtained from inedible vegetable oils (Renewable and Sustainable Energy Reviews 2011,131-140, Atomization and Sprays 2011,87-105), alcohols such as methanol, ethanol (submitted to Applied Energy) and butanol (Fuel 2016, 641-658), bio-oil (Renewable and Sustainable Energy Reviews 2014, 1108-1125), straight vegetable oil (Renewable and Sustainable Energy Reviews 2017, 80-97), BTL diesel, hydrotreated vegetable oils (Fuel 2014, 88-96).
Abstract:
According to the importance of methanol as an alternative biofuel and the current research trends towards more advanced internal combustion (IC) engine, it is required to fully understand the combustion and emission characteristics of advanced compression ignition (CI) engines fueled with methanol. Biomethanol can be produced from various biomass such as agricultural waste, forestry waste, livestock and poultry waste, fishery waste and sewage sludge through pyrolysis, gasification, biosynthesis and electrolysis etc. The main concern in this review is the application of biomethanol to advanced CI engines such as HCCI (homogenous charge compression ignition), PPC (partially premixed combustion), DF (duel fuel), RCCI (reactivity controlled combustion ignition) combustion mode. This review is a part of an on-going review project of application of bioalcohols to the advanced CI engines. In this review, it is found that the method for HCCI combustion in CI engine fueled with biomethanol can be divided into three categories: i.e. external, internal, and combined mixture preparation. DF combustion mode can be divided into four categories, i.e. blends, emulsion, fumigation, and dual fuel injection. In DF combustion mode, dual fuel injection can also be divided by two strategies, i.e. 1) PFI of the methanol and DI of the diesel in cylinder, 2) PFI of the methanol and DI of straight vegetable oil, Of two techniques, the methanol PFI (port fuel injection) and diesel DI (direct injection) was the prevailing technique to be studied in the dual fuel combustion. RCCI combustion mode can be divided into three categories, i.e. 1) methanol PFI/diesel or biodiesel DI, single injection, 2) methanol PFI/diesel DI, double injection, 3) methanol PFI/ diesel DI, triple injection.
Lunch Break 13:20-14:20 @ Restaurant Rienäcker
Brautsch Markus
Technical University of Applied Sciences Amberg Weiden, Germany
Title: Woodgas CHP units: an efficiency and system comparison of the dual-fuel and gas-otto engine combustion process
Time : 14:20-14:50
Biography:
Brautsch Markus is a Full Professor for thermodynamics, energy technology and renewable energies at the Technical University of Applied Sciences Amberg-Weiden, Germany since 1998. He is the Founder of the Institute of Energy Technology and the Bavarian Center of Excellence for Combined Heat and Power Generation. In 2014 he was appointed as Guest Professor at the Jiangsu University of Science and Technology in China. He is a Guest Lecturer at the Renewable Energy Center in Mithradam (India) and the University of Santa Caterina (Brazil).
Abstract:
A system comparison involving two gasifier-CHP systems (Dual Fuel and Gas-Otto) was conducted, with an emphasis on the efficiency of the complete systems. A complete system consists of a biomass gasifier and a CHP unit. The gasifier is composed of a wood pellet storage, a gasification chamber, a gas cooler, a gas filter and dust removal, as well as a condensing unit. The system is a direct-current fixed-bed gasifier with a localized fluidized bed. Wood pellets and gasification air are introduced into the gasifier from below in direct current. The gasification process is autothermal, meaning the thermal energy required for the gasification process comes from the partial combustion of the pellets during the process. After the gas has formed, the wood gas emerges at about 800°C at the upper end of the gasifier. It is cooled to about 125°C by means of a gas cooler. A downstream fabric filter cleans the raw gas of dust and ash particles. Downstream, the raw gas is cooled to 40°C by condensation of water. In Dual Fuel operation the CHP system works with a compression rate of 14:1. The electrical efficiency of the complete system at full load (180 kWel) varies from 34.4 % (heating oil/wood gas), (biodiesel/wood gas), (rapeseed oil/wood gas) to 33.3 % (palm oil/wood gas) and (soybean oil/wood gas) dependent on the used pilot fuel. The thermal efficiencies vary from 44.4 % to 48.7 %. As a result, the power coefficients amount from 69.8 % to 75.2 %. The λ values are constant with 1.53 to 1.57 and independent of used pilot fuels. Considering the additional heat output from the gas cooler of 75 kW, the gasifiers total efficiency is 91.2 %. For the gas-otto operation the CHP system has been modified to a reduced compression rate of 12.6:1. The whole injection system and cylinder head has been replaced to a cylinder head with ignition coils and spark plugs, so that the 100 % woodgas operation without any pilot fuel comes possible. The maximum electrical power was limited to 165 kW. The wood pellet mass flow has been constant with 108.7 kg/h, which correlates to a wood pellet combustion heat performance to 532.7 kW. The electrical efficiency of the wood gasifier CHP system is 29.9 %. Its thermal efficiency is 52.3 %. As a result, the power coefficient amounts to 0.57 (λ value of 1.55). The reduction of the compression ratio and the conversion to the gas-otto combustion process shows a decrease in electrical efficiency and power coefficient.
Bor-Yann Chen
National I-Lan University, Taiwan
Title: Deciphering synergistic characteristics of medicinal herbs and edible flora to stimulate electrochemically-steered biorefinery
Time : 14:50-15:20
Biography:
Bor Yann Chen has expertise in biomass energy and bioremediation for biotechnology. His serial studies focuses on applications in wastewater decolorization, bioremediation engineering, environmental toxicology and biofuel cells. Recently, his findings also deciphered chemical structures of electron shuttles and recalcitrant dyes which are crucial to simultaneous pollutant biodegradation and biomaterial/bioenergy recycling for sustainable green technology. Considering environmental friendliness, this study explored natural bioresources (e.g., medicinal herbs and edible flora) for bioenergy and high-value production generation. He has provided different alternatives to re-evaluate indigenous biomaterials with electrochemical potentials for bioenergy extraction, biorefinery development and derived applications.
Abstract:
Electron shuttle-stimulating microbial fuel cells is electrochemically promising to maximize performance of simultaneous wastewater treatment and bioproduct generation. Their prior studies revealed that bio decolorized intermediates owned capabilities as electron shuttles (ESs) to stimulate reductive decolorization and bioelectricity generation. Recent findings indicated that both antioxidant characteristics and electron-shuttling potential of chemical species were strongly associated. For medicinal herbs and edible flora, these properties are also directly proportional to contents of polyphenolics and/or flavonoids. Thus, this study quantitatively disclosed such relationships via electrochemical inspections for practicability. Moreover, the performance of bioelectricity generation using microbial fuel cells could be significantly augmented via supplementation of extracts of ES-rich medicinal herbs and edible flora. They also evaluated redox potential profiles (CV) and DPPH free radical scavenging capabilities of herbs or flora for the feasibility of bioelectrochemical applications. They also uncovered that extracts of Syzygium aromaticum, Lonicera japonica and green tea were promising ES-abundant herbs/flora for energy extraction/recycling. Due to reversible ES characteristics, wastes of medicinal herbs and edible flora were still feasible for reuse/recycling in electrochemically-steered applications to bioenergy and biorefinery.
Wen-ying LI
Taiyuan University of Technology, China
Title: Role of biomass during co-gasification of coal and biomass
Time : 15:20-15:50
Biography:
Wen Ying Li has her current research focused entirely on enabling discovery and design of processes and catalysts for sustainable energy, including converting coal to liquid and fuels, providing clean conversion technology from lower rank of coal and poor quality coal, clean and efficient catalytic pyrolysis and gasification of lignite and biomass, and optimizing traditional coal conversion processes and integrating carbon-based polygeneration system of carbon mitigation initiative.
Abstract:
This article academically discusses the role of biomass during the co-gasification of coal and biomass, according to the effects of addition ratio of biomass, biomass ash, alkali metal compounds in biomass ash, and mineral matters in the coal on anthracite char gasification under CO2 atmosphere. The transformation of organic structure and mineral matter in coal-biomass mixtures during co-gasification, the anthracite and rice straw addition with different ratios were isothermally gasified at 1100°C. The phase-mineral composition, morphology and organic structure of solid residues produced at different gasification time were analyzed by X-ray diffraction, scanning electron microscopy coupled with energy dispersive spectrometer, Raman spectroscopy and other methods. Results revealed that the organic structure was changed in char as it became less ordered with the addition of biomass. The bulk concentrations of K and Na and their bearing minerals and phases in char increased with the addition of biomass during gasification process. The transformation of mineral matter played a significant role in promoting the coal gasification. Biomass ash containing alkali metals has been proven as a natural and disposable catalyst for the thermal conversion of carbon-containing material. Meanwhile, it was observed that 50% biomass ash addition resulted in the agglomeration of the co-gasification ash. The catalytic effect of alkalis in biomass ash was attributed to the H2O soluble and HCl insoluble forms alkali metal containing chemicals during gasification process. Catalytic activity of 2.5% biomass ash addition to demineralized coal char is similar to the 30% biomass ash addition to coal char. The mineral matter in the coal was observed to decrease the catalytic activity of the biomass ash which could be partially remedied by calcium additives. The catalytic mechanism of biomass ash on coal char gasification was elucidated. We researched the fusion process from sintering to melting of anthracite coal ash, rice straw ash and their mixture with different rice straw ash additions. Two different fusion mechanisms were applied to elucidate the fusion process with the increment of rice straw ash addition. The above results can be used in the development of coal-biomass co-gasification technology.
Networking and Refreshments Break 15:50-16:20 @ Sylt Foyer
Tanja Radu
Loughborough University, UK
Title: PhytoPower: Safely transforming mercury phytoremediation crops into bioenergy
Time : 16:20-16:50
Biography:
Tanja Radu is a Lecturer in International Relief, Water Supply and Sanitation Engineering at Loughborough University, UK. She has more than 15 years of international experience in water and environmental engineering. Her main research interests include waste water treatment, renewable energy from waste and supplying energy for rural communities in developing countries. Currently, she is focusing on the process of biogas generation from waste using the technology of anaerobic digestion. She is involved in a range of international projects providing small-scale, decentralized sustainable energy generation. This includes collaborative effort with Universities of India, Thailand and Bahrain.
Abstract:
Statement of the Problem: The Minamata Convention on Mercury entered into force in August 2017 and seeks to redress mercury contamination across its 128 signatories and 84 ratified parties. Some 5,500-8,900 tonnes of mercury are released into the biosphere annually negatively affecting the health of hundreds of thousands of people worldwide. Cleaning up contaminated site using standard methods is expensive and unrealistic in many affected areas, primarily in developing countries. Developing a more cost-effective method that includes co-benefits is needed.
Methodology & Theoretical Orientation: The research team worked with an industry partner to test its patented method for site decontamination. The method uses plants to take up heavy metal pollution (called phytoremediation), decontaminates phytoremediation biomass, and uses treated biomass as a feedstock for anaerobic digestion. This is a novel method of coupling land remediation with renewable energy generation using plants. Mercury distribution throughout this system is monitored with the special emphasis on efficiency of its removal from every step of the cycle using a novel polymer (adsorbent). Systematic analysis of soil, plant, and AD (anaerobic digestion) digester samples indicates sinks of mercury and the optimal conditions for its adsorption. The experimental work includes using real soil and plant samples from mercury contaminated sites in Indonesia. Several types of plants are studied to provide maximal mercury uptake and biogas yield.
Conclusion & Significance: The research shows the efficacy of the method to remove mercury from contaminated biomass, and the efficiency of treated biomass when used as an AD feedstock (typically mercury interferes with the AD process). It is significant because the method could be applied to vast tracts of contaminated land to support site remediation whilst creating a bioenergy value stream from currently poisoned land. The method complies with Minamata Convention provisions and could significantly improve the health and welfare of people especially in developing countries.
Knawang Chhunji Sherpa
Indian Institute of Technology, India
Title: PhytoPower: Safely transforming mercury phytoremediation crops into bioenergyAn evaluation of fermentation approaches for ethanol production from enzymatically pretreated sugarcane tops
Time : 16:50-17:10
Biography:
Knawang Chhunji Sherpa is currently pursuing her PhD at PK Sinha Centre for Bioenergy at the Indian Institute of Technology, Kharagpur, India. Her research work is focused on second generation bioethanol using sugarcane tops as lignocellulosic biomass.
Abstract:
Second generation bioethanol has been advocated as a promising substitute of petroleum based fuels for mitigating GHG emissions and lessening our dependency on fossil based fuels. Bioethanol has emerged as one of the advantageous sustainable biofuel that aids in being an effective factor in the transportation sector for reducing emission of pollutants from tailpipe that are the reason for smog and ground-level ozone. Bioethanol due to its high octane number of 108 has high anti-knock value and can be used in bioethanol-diesel blend to decrease exhaust gas emission. In addition, bioethanol is less noxious producing less air-borne pollutants in comparison to petroleum fuel. Typical process for the biological conversion of carbohydrates to ethanol comprises of pretreatment, saccharification and fermentation. Development in fermentation technology plays a significant role in making the process viable. Fermentation being a key element in the bioethanol production process, the present study investigates different strategies viz. separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF) and semi-simultaneous saccharification and fermentation (SSSF) to produce ethanol from sugarcane tops enzymatically pretreated with laccase. Sugarcane tops, an agricultural residue was used as the substrate since it is rich in carbohydrates that are usually burnt in the field or used as low quality roughage. The focus of the study was to check the efficiency of various approaches among which SSF and SSSF were able to enhance ethanol titre in the range of 6-7 % (v/v) with shortened biological processing time (24-36 h).
G. Lohit K. Srinivas
Indian Institute of Technology, India
Title: Green catalyst mediated biodiesel production from waste cooking oil
Biography:
G Lohit K Srinivas is currently a PhD Scholar at the Indian Institute of Technology Kharagpur, India. His area of research include: biodiesel production utilizing oleaginous microbes.
Abstract:
Waste cooking oil generated as a waste product during the manufacture of fried foods poses grave health risk to its consumers owing to the change in the chemical and physical properties of the oil during the frying process. In this regard, waste cooking oil can be a potential resource for biodiesel production owing to its abundant availability and moreover its utilization will curb the problem of its disposal and thereby negating the pollution incurred towards water and land resources. The availability of waste cooking oil depends upon the quantity of the consumption of edible oil and according to EIA (environmental impact assessment), in the United States of America, availability of waste cooking oil is estimated to be 100 million gallons per day. Major fraction of the waste cooking oil is dumped in the landfills thus leading to environmental problems while a small fraction is utilized for soap manufacturing and also as an additive during the manufacture of animal fodder which is also under the scanner owing to the European Union regulation of 2002 according to which its use in the manufacture of animal fodder is banned. Thus the route of biodiesel production from waste cooking oil is a potential value addition to this resource. Conventionally biodiesel is manufactured via transesterification of the lipids to yield fatty acid methyl esters utilizing acid, alkali etc., catalysts which pose the issue of corrosion to the reactors as well as the waste disposal issues. In this regard, utilization of lipase extracted from Rhizopus oryzae was investigated for transesterification of waste cooking oil to FAME (Fatty Acid Methyl Esters) and it was observed that the biodiesel produced majorly composed of palmitic and stearic acid methyl esters. Moreover the fuel quality of the produced biodiesel yielded calorific value of 37.83 MJ/kg, acid value of 0.2 mg KOH/ g biodiesel, iodine value of 8.71 g I2/ 100 g biodiesel and cetane index of 67.3. The characteristics studied fall well within the ASTM D6751 and EN 14214 standards prescribed for biodiesel thus justifying the use of green catalysts – lipase for biodiesel production of waste cooking oil.